Spectrophotometric Determination of Vitamin B6 in Pharmaceutical Formulations and Wastewater Samples
Nief Rahman Ahmad* and Ghfran Naif Rahman
Abstract
A simple, accurate and rapid sensitive indirect method for determination of Vitamin B6 (pyridoxine hydrochloride) has been developed. The proposed method is based on formation of a colored complex by the reaction between released SCN ion from Hg (SCN)2 and ferric ions to form red soluble product with maximum absorption at 460 nm. Beer’s law is obeyed over the concentration range of 2-28µg/ml, with molar absorptivity and Sand ell s sensitivity of 0.729x104l/mol.cm. and 0.0281µg.cm-2 respectively, relative standard deviation (RSD)is less than1.6 (n=10). The method is applied successfully for determination of Vitamin B6 in some pharmaceutical preparations (injection and tablets) and wastewater samples. The present method is considered to be simple because it does not need either heating or hydrolysis or solvent extraction steps. The ingredients often formulated with Vitamin B6 have been shown not to interfere, and the proposed method is suitable for the routine determination of Vitamin B6.
