Synthesis of Silver Nanoparticles Using Plant Extracts from Legeneraria Siceraria Fruits and Assessment of their Anti-Bacterial and Anti-Oxidant Activities
Getu Asnake Fentaye and Gamada Begna Sisay*
ABSTRACT
Nanotechnology is a key subject of science, engineering, and technology that is experiencing enormous growth in the current century. In this study, fruits of the legeneraria siceraria plant, this is widely used in traditional medicines in Ethiopian rural areas, used as reducing and capping agent for AgNPs synthesis. The legeneraria siceraria fruits -mediated synthesized AgNPs characterized by ultraviolet-visible (UV-VIS.) spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy, X-ray diffractometer (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS). The optimal synthesization parameters for AgNPs in this study were temperature 60 °C, stirring time 60 minutes, pH 11, and 2 mM silver nitrate concentration. Characteristic and well-defined SPR bands for AgNPs were confirmed at around λmax = 426 nm. Synthesized AgNPs of calculated mean crystallite size were 10.21 nm. Scanning electron microscopy (SEM) revealed the synthesized AgNPs have a spherical structure. The antibacterial activity of AgNPs synthesized using legeneraria siceraria fruits medicinal plants demonstrated that Gram-positive and Gram-negative bacteria inhibited. Additionally, the AgNPs showed minimal toxicity to human cells. AgNPs were useful antioxidants for health preservation against different oxidative stress associated with degenerative diseases. In this work, it acts at scavenging against DPPH.
