20 Years Experiences on Paprika (Capsicum annuum L.) Doubled Haploid Production
Aniko Gémes Juhász* and Zsolt Sági
ABSTRACT
In laboratory of Medimat Ltd. over the past twenty years have been tested more than 3600 different genotypes originating from Hungarian sweet pepper types (Cecei, tomato-shaped, apple-shaped, white blocky, light green and dark green blocky, green spice), Hungarian spice pepper genotypes, Dutch blocky types, Spanish types (Dolce Italiano, Lamuyo, red blocky, yellow blocky), Chinese types (hot lamuyo and different spice types) and Turkish types (e.g. Dolma, Charliston, Sivri) using in vitro anther and microspore culture to produce new homozygote breeding lines for the private companies.
We improved a very efficient genome doubling method by treating the haploid paprika regenerants with colchicine (500 mg/l) in vitro for six days. The ratio of induced doubled haploids reached to 95.13 %. Survival rate of treated haploid plants was 99.4 %. Since diploidised plants developed similarly to their untreated, spontaneous diploid counterparts and since no fertility problems were recorded in the past fifteen years.
When haploid method has been combined with marker-based pre-selection it provided an exceptionally efficient method for paprika resistance breeding (TMV, TSWV, powdery mildew, Xanthomonas, nematodes).
